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fluorescence microscopy

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Series: ASM Technical Books
Publisher: ASM International
Published: 01 November 2010
DOI: 10.31399/asm.tb.omfrc.t53030089
EISBN: 978-1-62708-349-2
... the paint layer (10× objective). (b) A fluorescent penetration dye (Magnaflux Zyglo) was applied on the surface of the specimen to enhance the contrast of the microcracks. Epi-fluorescence, 390–440 nm excitation, 10× objective Abstract The analysis of composite materials using optical microscopy...
Series: ASM Technical Books
Publisher: ASM International
Published: 01 November 2010
DOI: 10.31399/asm.tb.omfrc.t53030177
EISBN: 978-1-62708-349-2
...-fluorescence. First, the sample was immersed in a solution of Rhodamine B dye (see Chapter 5, “Viewing the Specimen Using Reflected-Light Microscopy,” for dyes). The sample was then ultrasonically cleaned in methylene chloride, followed by heating to 121 °C (250 °F) to remove additional solvent. Using epi...
Series: ASM Technical Books
Publisher: ASM International
Published: 01 June 2016
DOI: 10.31399/asm.tb.hpcspa.t54460121
EISBN: 978-1-62708-285-3
...-Ray photoelectron spectroscopy, X-ray fluorescence, Auger electron spectroscopy, Raman spectroscopy, oxygen analysis, and nanoindentation. cold-sprayed coatings residual-stress analysis hardness bond adhesion strength microscopy spectroscopy diffraction MATERIALS CHARACTERIZATION...
Series: ASM Technical Books
Publisher: ASM International
Published: 01 December 2018
DOI: 10.31399/asm.tb.fibtca.t52430107
EISBN: 978-1-62708-253-2
... Abstract This chapter describes some of the most effective tools for investigating boiler tube failures, including scanning electron microscopy, optical emission spectroscopy, atomic absorption spectroscopy, x-ray fluorescence spectroscopy, x-ray diffraction, and x-ray photoelectron...
Series: ASM Technical Books
Publisher: ASM International
Published: 01 November 2010
DOI: 10.31399/asm.tb.omfrc.t53030193
EISBN: 978-1-62708-349-2
...-dyed epoxy casting resin. The use of epi-bright-field illumination does not allow the dye to fluoresce, and therefore, the cracks are hard to distinguish. Bright-field montage, 5× objective Fig. 11.4 Impact damage of a carbon fiber composite material that has a toughened matrix. (a) Montage...
Series: ASM Technical Books
Publisher: ASM International
Published: 01 December 2009
DOI: 10.31399/asm.tb.sfa.t52780193
EISBN: 978-1-62708-268-6
... Visual examination, x-ray or n-ray (if device cannot be disassembled and depending on materials) Internal component presence and fit Visual examination, x-ray or n-ray (if device cannot be disassembled and depending on materials) Contamination Visual examination, magnification, optical microscopy...
Series: ASM Technical Books
Publisher: ASM International
Published: 01 November 2010
DOI: 10.31399/asm.tb.omfrc.t53030159
EISBN: 978-1-62708-349-2
... to fatigue cycling. bright-field illumination composite materials epi-fluorescence dark-field illumination microcrack analysis polarized light microscopy ...
Series: ASM Technical Books
Publisher: ASM International
Published: 01 November 2010
DOI: 10.31399/asm.tb.omfrc.t53030023
EISBN: 978-1-62708-349-2
... on the microscopist and the capability of the microscope used. The polished cross-sectional mounts can be examined with a variety of microscopy techniques, including polarized light, bright- and dark-field illumination, and epi-fluorescence. The viewing of dyed specimens is discussed in Chapter 5, “Viewing...
Book Chapter

Series: ASM Technical Books
Publisher: ASM International
Published: 01 December 1984
DOI: 10.31399/asm.tb.mpp.t67850267
EISBN: 978-1-62708-260-0
... light and orthochromatic film. Achromats are useful in fluorescence microscopy, since fluorite components in more highly corrected objectives will autofluoresce. Achromats generally have relatively large working distances (distance from front of lens to sample) and are useful in hot-stage microscopy...
Series: ASM Technical Books
Publisher: ASM International
Published: 01 November 2010
DOI: 10.31399/asm.tb.omfrc.t53030245
EISBN: 978-1-62708-349-2
... material in place, minimizing artifacts. The specimen can be remounted to provide adequate handling of the microscopic sample. Polished cross-sectional mounts can be examined with a variety of microscopy techniques, including polarized light, bright- and dark-field illumination, and epi-fluorescence...
Book Chapter

Series: ASM Technical Books
Publisher: ASM International
Published: 01 December 2009
DOI: 10.31399/asm.tb.sfa.t52780093
EISBN: 978-1-62708-268-6
.... Techniques in this area include visual examination, low-power magnification, optical and scanning electron microscopy, and photography. Dimensional inspection and related approaches for assessing suspected item dimensions and conformance to drawing and specification requirements and determining...
Series: ASM Technical Books
Publisher: ASM International
Published: 01 November 2019
DOI: 10.31399/asm.tb.mfadr7.t91110209
EISBN: 978-1-62708-247-1
... sections are devoted to the discussion on liquid-crystal imaging and fluorescent microthermal imaging technique for thermal detection. defect localization failure analysis fluorescent microthermal imaging infrared thermography liquid-crystal imaging thermal detection techniques Introduction...
Book Chapter

Series: ASM Technical Books
Publisher: ASM International
Published: 01 April 2013
DOI: 10.31399/asm.tb.imub.t53720139
EISBN: 978-1-62708-305-8
...: Ref 3 Physical Basis Auger electrons are emitted as the excited atoms relax. In a sense, they are the complements of the characteristic x-rays that are used for chemical characterization in x-ray fluorescence (XRF), SEM, EPMA, and transmission electron microscopy (TEM). When atoms become...
Book Chapter

Series: ASM Technical Books
Publisher: ASM International
Published: 01 December 2018
DOI: 10.31399/asm.tb.fibtca.t52430325
EISBN: 978-1-62708-253-2
... with a thin adherent magnetite scale that was grayish black in color. Fig. 6.137 Portion of a failed superheater tube showing failure location Wet fluorescent magnetic particle inspection studies indicated hairline cracks emanating from the corner of the rupture contours ( Fig. 6.138a...
Series: ASM Technical Books
Publisher: ASM International
Published: 01 November 2010
DOI: 10.31399/asm.tb.omfrc.9781627083492
EISBN: 978-1-62708-349-2
Series: ASM Technical Books
Publisher: ASM International
Published: 01 November 2019
DOI: 10.31399/asm.tb.mfadr7.t91110434
EISBN: 978-1-62708-247-1
...-reflection x-ray fluorescence (TXRF). Each of these capabilities is given for “ideal” conditions, and optimum sensitivity and lateral resolution may not be achievable at the same time. Table 1 Properties of analytical techniques including energy dispersive x-ray spectroscope (EDS) in the SEM or STEM...
Series: ASM Technical Books
Publisher: ASM International
Published: 01 November 2010
DOI: 10.31399/asm.tb.omfrc.t53030001
EISBN: 978-1-62708-349-2
... Materials and Optical Microscopy / 11 Fig. 1.9 Thermoplastic stitch in carbon fiber composite material. Note the microcracks in the center of the stitch. Epi-fluorescence, 390 440 nm excitation, 25× objective 1.10a to 1.10c) (Ref 11, 12). The most common rubber materials used for toughening thermoset...
Book Chapter

Series: ASM Technical Books
Publisher: ASM International
Published: 01 October 2005
DOI: 10.31399/asm.tb.faesmch.t51270025
EISBN: 978-1-62708-301-0
... and electron microscopy. It presents a number of images recorded using these methods and explains what they reveal about the mode of fracture and the state of the component prior to failure. fractography microscopic examination macroscopy IN FAILURE ANALYSIS, examination is done at various levels...
Series: ASM Technical Books
Publisher: ASM International
Published: 01 October 2005
DOI: 10.31399/asm.tb.faesmch.t51270019
EISBN: 978-1-62708-301-0
...%; accuracy, 2 to 5% X-ray fluorescence analysis: Normally applicable to elements heavier than sodium; accessible range, 0.005 to 10%; accuracy, 2 to 5% Techniques for Local Composition Variations Laser probe microanalysis: Applicable to nearly all elements; accessible range, 0.01 to 100...
Series: ASM Technical Books
Publisher: ASM International
Published: 01 July 1997
DOI: 10.31399/asm.tb.wip.t65930039
EISBN: 978-1-62708-359-1
... of an indicator fluid that has a surface tension sufficiently low to be drawn into surface cracks too small to be detected visually. The excess dye or fluorescent material is then removed from the surface, but it remains in and highlights the cracks when a developer is applied. Figure 3 shows cracks in a flux...