Failure localization is one of the vital processes in the field of failure analysis. However, as newer fabrication processes emerge and demand for smaller transistors keeps on increasing, the complexity of failure analysis fault isolation involving micro-probing also increases along with the challenges on fault isolation equipment such as limited magnification and susceptibility to vibrations. In this paper, the capability of Focused Ion Beam (FIB) to perform circuit edit was utilized along with Avalon CAD navigation to pinpoint the location of the defects without the need of micro-probing while doing fault isolation. Results showed that through this technique, physical defect locations were successfully identified in three different case studies.