The velocity of particles prior to the impact on the substrate surface is a very important factor that determines the deposition characteristics and coating quality in cold spray. The DPV-2000 system is an on-line diagnostic system that simultaneously measures the velocity, temperature and diameter of thermally sprayed particles. In the present study, the DPV-2000 system was employed to measure the velocity and diameter of cold sprayed particles. The effects of pre-setting software parameters of the DPV-2000 system on the measured particle velocities were investigated. The experimental results showed that the pre-setting values of the software parameters produced a significant influence on the accuracy of measured results.