Cold sprayed copper coatings have been widely studied but the effect of substrate properties and spraying conditions on the bond strength still needs further investigations. Using nanoindentation, it is possible to compare the effect of spray parameters on the change in hardness of the coating due to the difference in size distribution of the powder. Deposition of 2 copper powders on aluminium and Ta6V substrates has been done at different stagnation temperatures and pressures using nitrogen as process gas. Hardness profiles show how the speed of the particles induces shot peening effects on the surface. The interaction between the hot gas jet and the surface may modify the hardness profiles. The effect on the substrate hardness has also been investigated.