Abstract With semiconductor geometries approaching sub 10 nanometer gate levels in the not too distant future and with higher levels of integration, new ways of characterizing defects and examining the 3D distribution visually and elementally on the nanometer level are required to keep up with the demands of the modern FA lab. The purpose of this study is to demonstrate the workflow and show the results of an automated 3D STEM-EDX data acquisition & visualization system that can be utilized for the failure analysis of semiconductor devices.