With increasing complexity involved in advance node semiconductor process development, dependability on parametric test structures has also increased significantly. Test structures play a predominant role throughout the entire development cycle of a product. They are used to understand the process windows and also help to monitor the health of a line. This work provides a process flow sheet for root cause identification on chain opens on advanced 20 nm and sub-20 nm technologies setting a standard guideline for a specific category fail type. It provides a consistent way of attack in a much more streamlined fashion. Further, dependability on TEM rather than convention FIB cross-sections provides shortest time to root cause identification. Three typical cases encountered are discussed to demonstrate the idea: embedded chain opens by electron beam absorbed current (EBAC) isolation, chains opens at level by EBAC isolation, and chains opens at level by passive voltage contrast isolation.