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transmitted light microscopy

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Series: ASM Handbook
Volume: 9
Publisher: ASM International
Published: 01 December 2004
DOI: 10.31399/asm.hb.v09.a0009076
EISBN: 978-1-62708-177-1
...Abstract Abstract This article describes the various aspects relating to the selection and preparation of ultrathin-section specimens of fiber-reinforced polymeric composites for examination by transmitted light microscopy. It provides information on the contrast-enhancement methods used...
Series: ASM Handbook
Volume: 21
Publisher: ASM International
Published: 01 January 2001
DOI: 10.31399/asm.hb.v21.a0003464
EISBN: 978-1-62708-195-5
..., and epi-fluorescence. The article also provides information on transmitted light microscopy. composite materials epi-bright-field illumination epi-dark-field illumination epi-fluorescence epi-polarized light failure analysis illumination methods mounting polishing quality control reflected...
Series: ASM Handbook
Volume: 9
Publisher: ASM International
Published: 01 December 2004
DOI: 10.31399/asm.hb.v09.a0009082
EISBN: 978-1-62708-177-1
...-matrix composites, transmitted polarized-light microscopy can be used very effectively. The preparation of thermoplastic-matrix composites for optical microscopic analysis requires the creation of ultrathin sections so that transmitted polarized light can be used. Throughout the literature, this has...
Series: ASM Handbook
Volume: 9
Publisher: ASM International
Published: 01 December 2004
DOI: 10.31399/asm.hb.v09.a0009094
EISBN: 978-1-62708-177-1
..., “Special Sample Preparation and Polishing,” D. Jean Ray for her contributions to the article, “Thin-Section Preparation and Transmitted Light Microscopy,” and Anthony Falcone for his contributions to the article “The Effects of Lightning Strikes on Polymeric Composites.” ...
Series: ASM Handbook
Volume: 9
Publisher: ASM International
Published: 01 December 2004
DOI: 10.31399/asm.hb.v09.a0009075
EISBN: 978-1-62708-177-1
... differences arise from relief on the specimen surfaces. Fluorescence Microscopy Epi-fluorescence microscopy uses an ultraviolet light source (mercury or xenon) for illumination. The light is transmitted though an excitation filter that blocks all but a narrow bandwidth of the light spectrum. The narrow...
Series: ASM Handbook
Volume: 9
Publisher: ASM International
Published: 01 December 2004
DOI: 10.31399/asm.hb.v09.a0009083
EISBN: 978-1-62708-177-1
... this analysis, an ultrathin section (0.5 to 5 μm) must be developed from the honeycomb sandwich structure composite part to be able to use all of the contrast techniques of transmitted-light microscopy. Figure 1 shows an area of a honeycomb-cored sandwich structure composite cross section that is viewed...
Series: ASM Handbook
Volume: 9
Publisher: ASM International
Published: 01 December 2004
DOI: 10.31399/asm.hb.v09.a0009080
EISBN: 978-1-62708-177-1
... of the thermosetting resin. As a result, the phase separation of the rubber may be incomplete, which can lead to ambient- and elevated-temperature property degradation. In the following figures, ultrathin sections were developed from the composite materials to use transmitted-light optical microscopy contrast...
Series: ASM Handbook
Volume: 9
Publisher: ASM International
Published: 01 December 2004
DOI: 10.31399/asm.hb.v09.a0003753
EISBN: 978-1-62708-177-1
..., while transmitted light microscopy can be more effectively used in the microstructural imaging of polymers (where specimens may allow more light transmission). The choice of method also depends on imaging requirements such as resolution, magnification, depth of field, and lens aberration. These factors...
Series: ASM Handbook
Volume: 9
Publisher: ASM International
Published: 01 December 2004
DOI: 10.31399/asm.hb.v09.a0009072
EISBN: 978-1-62708-177-1
... Preparation and Transmitted-Light Microscopy” ), this mold geometry is necessary. Summary of Mounting Procedure The following steps are recommended to achieve a good mounted specimen without voids or specimen pull-out: Select a mold to hold the specimens, and coat with a mold release agent...
Series: ASM Handbook
Volume: 9
Publisher: ASM International
Published: 01 December 2004
DOI: 10.31399/asm.hb.v09.a0009081
EISBN: 978-1-62708-177-1
... on the matrix microstructure, including matrix strains and fracture morphology, can be determined with the development of ultrathin sections and transmitted polarized light or one of many other contrast methods. Through the use of these techniques, a complete analysis of the damage response of fiber-reinforced...
Series: ASM Handbook
Volume: 9
Publisher: ASM International
Published: 01 December 2004
DOI: 10.31399/asm.hb.v09.a0009071
EISBN: 978-1-62708-177-1
... in.) macrophotograph Fig. 16 Ultrathin section showing the microstructure of spruce wood. Transmitted-light phase contrast, 40× objective Optical Microscopy of Composite Materials Optical microscopy is a valuable tool in materials investigations related to problem solving, failure analysis...
Series: ASM Desk Editions
Publisher: ASM International
Published: 01 December 1998
DOI: 10.31399/asm.hb.mhde2.a0003252
EISBN: 978-1-62708-199-3
... of light microscopy are covered in the Section “Metallography” and will not be repeated here. While light microscopy is an important metallurgical tool, it has a number of limitations: Spatial resolution: Conventional light microscopes cannot resolve features smaller than ∼1 μm. Depth of field...
Series: ASM Handbook
Volume: 10
Publisher: ASM International
Published: 15 December 2019
DOI: 10.31399/asm.hb.v10.a0006681
EISBN: 978-1-62708-213-6
... Visible light 390–760 200 0.00251 Ultraviolet ray 13–390 400 0.00164 X-ray 0.05–10 1000 0.00087 γ-ray 0.005–0.1 … … Source: Ref 1 Features of electron sources used in transmission electron microscopy Table 2 Features of electron sources used in transmission electron...
Series: ASM Handbook Archive
Volume: 10
Publisher: ASM International
Published: 01 January 1986
DOI: 10.31399/asm.hb.v10.a0001766
EISBN: 978-1-62708-178-8
... a transmission electron microscope is analogous to the visible light ray path through the ground glass lenses in an optical microscope configured to operate in the transmitted-light mode (except that the electron follows a spiral path through the lens as it is focused, while light is focused through the lens...
Series: ASM Handbook
Volume: 9
Publisher: ASM International
Published: 01 December 2004
DOI: 10.31399/asm.hb.v09.a0003788
EISBN: 978-1-62708-177-1
..., their characteristics and sample preparation procedures. It reviews the methods pertaining to the microstructural examination of bulk magnetic materials, including microscopy techniques specified to magnetic materials characterization, with specific examples. The techniques used in the study of magnetic domain...
Series: ASM Handbook
Volume: 9
Publisher: ASM International
Published: 01 December 2004
DOI: 10.31399/asm.hb.v09.a0009085
EISBN: 978-1-62708-177-1
.... Further preparation of the reflected-light sample to an ultrathin-section sample revealed more information about the heat generation created by the lightning strike. The vaporized and degraded matrix in the first ply can easily be seen by using transmitted-light optical microscopy. Deeper under the damage...
Series: ASM Handbook
Volume: 9
Publisher: ASM International
Published: 01 December 2004
DOI: 10.31399/asm.hb.v09.a0003760
EISBN: 978-1-62708-177-1
...Abstract Abstract Three-dimensional microscopy can be used to reveal the shape, distribution, and connectivity of three-dimensional (3D) features that lie buried within an opaque material. This article discusses several experimental techniques that can be used to generate 3D images...
Series: ASM Handbook
Volume: 9
Publisher: ASM International
Published: 01 December 2004
DOI: 10.31399/asm.hb.v09.a0003754
EISBN: 978-1-62708-177-1
... microscope, to obtain additional information, are also described. The article concludes with information on photomicroscopy and macrophotography. bright-field illumination dark-field illumination depth of field interference-contrast illumination light microscopy macrophotography mechanical...
Series: ASM Handbook
Volume: 23
Publisher: ASM International
Published: 01 June 2012
DOI: 10.31399/asm.hb.v23.a0005685
EISBN: 978-1-62708-198-6
... microscopy for the analysis of materials and device components generally refers to reflected light microscopy. In this method, light is reflected from the sample surface through the microscope objective to an eyepiece, view screen, or camera. Transmitted light is occasionally used for transparent...
Series: ASM Desk Editions
Publisher: ASM International
Published: 01 November 1995
DOI: 10.31399/asm.hb.emde.a0003057
EISBN: 978-1-62708-200-6
... include reflected light microscopy using polarized light, scanning electron microscopy, transmission electron microscopy, energy dispersive analysis of X-rays, and wavelength dispersive analysis of X-rays. Macroscopic property characterization involves measurement of porosity, density, and surface area...