Due to its layer-by-layer process, 3D printing enables the formation of complex geometries using multiple materials. Three-dimensional printing for bone tissue engineering is called bioprinting and refers to the use of material-transfer processes for patterning and assembling biologically relevant materials, molecules, cells, tissues, and biodegradable biomaterials with a prescribed organization to accomplish one or more biological functions. Currently, 3D bioprinting constructs can be classified into two categories: acellular and cellular. This article introduces and discusses these two approaches based on the suitable materials for these constructs and the fabrication processes used to manufacture them. The materials are grouped into polymers, metals, and hydrogels. The article also summarizes the commonly used 3D printing techniques for these materials, as well as cell types used for various applications. Lastly, current challenges in tissue engineering are discussed.

Hydroxyapatite (HA) is one of the most popular materials in tissue scaffold engineering due to its similarity to the nature of human bone; it accounts for more than half of the total weight of the latter. Selective laser sintering (SLS) is an additive manufacturing method that is used in producing tissue engineering parts from HA feedstocks. This article provides a brief overview of the process itself, along with a detailed review of HA-based tissue engineering applications using SLS. Discussion on the various polymer composites is presented. A detailed overview of selected publications on HA-based SLS studies is listed, which provides insight regarding technical aspects of processing HA powder feedstocks.

The field of bioprinting is a subset of additive manufacturing (AM) that is rapidly expanding to meet the needs of regenerative medicine and tissue engineering. Bioprinting encompasses a broad spectrum of issues, from cell expansion and novel bioink development to cell/stem cell printing, from organoid-based tissue organization to bioprinting of human-scale tissue structures, and from building cell/tissue/organ-on-a-chip to biomanufacturing of multicellular engineered living systems. This article focuses on two challenges regarding bioprinting: bioinks and crosslinking. It describes the methods for characterizing the performance of bioink formulations and the effectiveness of crosslinking strategies. The topics covered include modalities of bioprinting, characteristics of bioink, rheological properties of bioink sols, rheological measurements, mathematical models of bioink rheology, postfabrication polymer network mechanics, mechanical properties of crosslinked bioinks, and printability of bioinks. Finally, specific strategies used for crosslinking bioinks, as well as some emerging strategies to further improve bioinks and their crosslinking, are summarized.

This article outlines the selection criteria for choosing an implant material for biomedical devices in orthopedic, dental, soft-tissue, and cardiovascular applications. It details the development of various implants, such as metallic, ceramic, and polymeric implants. The article discusses specific problems associated with implant manufacturing processes and the consequent compromises in the properties of functionally graded implants. It describes the manufacturing of the functionally-graded hip implant by using the LENS process. The article reviews four different types of tissue responses to the biomaterial. It discusses the testing methods of implant failure, such as in vitro and in vivo assessment of tissue compatibility.

This article begins with a description of extrusion-based bioprinting for tissue scaffold fabrication. It also examines various extrusion-based bioprinting processes and related tissue scaffolding strategies, presents the selection criteria of various bioinks with various polymers and their printed scaffolds for applications in tissue engineering and regenerative medicines, and provides future research recommendations to address the shortcomings and issues found in current extrusion-based bioprinting processes.

Piezoelectric jetting is a common form of additive manufacturing technology. With the development of material science and manufacturing devices, piezoelectric jetting of biomaterials has been applied to various fields including biosensors, tissue engineering, deoxyribonucleic acid (DNA) synthesis, and biorobots. This article discusses the processes involved in piezoelectric jetting of biosensors and biorobots and the applications of piezoelectric jetting for tissue engineering and producing DNA. In addition, it reviews the challenges and perspectives of piezoelectric jetting.

Microvalve jetting, with its advantages of low cost, ease of operation, high printing speed, and ability to process living cells with high viability, has been primarily used for fabricating high-throughput drug-screening models, in vitro cellular structures for fundamental cell biology research, and cell-laden structures for regenerating tissues or organs in the human body after disease or trauma. This article provides an overview of microvalve jetting of biomaterials, including operational parameters. The jetting technologies covered are inkjet printing, microvalve jetting, and laser-assisted jetting. The parameters covered include nozzle size (nozzle inner diameter), pneumatic pressure, valve-opening time, and printing speed of microvalve jetting. Subsequently, the article discusses biomaterials for microvalve jetting in terms of biomaterial definition, required properties for a suitable biomaterial, currently used biomaterials, and cells and cellular structures. Additionally, applications of microvalve jetting in biomedical engineering are presented, which include cellular and RNA analysis, high-throughput drug screening, and tissue engineering.

Ceramics are used widely in a number of different clinical applications in the human body. This article provides a brief history of the bioceramics field and discusses the classification of bioceramics. These include bioinert ceramics, bioactive ceramics, and bioresorbable ceramics. The article describes third-generation bioceramics, classified by Hench and Polak, such as silicate-substituted hydroxyapatite and bone morphogenic protein-carrying calcium phosphate coatings. It reviews several examination methods used to test the biocompatibility of ceramics, namely, biosafety testing, biofunctionality testing, bioactivity testing, and bioresorbability testing.

In the field of medical device development and testing, the corrosion of metallic parts can lead to significant adverse effects on the biocompatibility of the device. This article describes the mechanisms of metal and alloy biocompatibility. It reviews the response of implant metals and particulate materials to corrosion. The effect of metal ions from an implanted device on the human body is also discussed. The article concludes with information on the possible cancer-causing effects of metallic biomaterials.

This article focuses on ceramics, glasses, glass-ceramics, and their derivatives, that is, inorganic-organic hybrids, in the forms of solid or porous bodies, oxide layers/coatings, and particles with sizes ranging from nanometers to micrometers, or even millimetres. These include inert crystalline ceramics, porous ceramics, calcium phosphate ceramics, and bioactive glasses. The article discusses the compositions of ceramics and carbon-base implant materials, and examines their differences in processing and structure. It describes the chemical and microstructural basis for their differences in physical properties, and relates the properties and hard-tissue response to particular clinical applications. The article also provides information on the glass or glass-ceramic particles used in cancer treatments.

This article discusses the mechanisms of metal and alloy biocompatibility. It provides information on early testing and experience with metals in medical device applications. The article describes the response of implant and particulate materials to severe corrosion. It provides a description of metal binding and its effects on metabolic processes. Hypersensitive responses to metal ions are also reviewed. The article concludes with a discussion on the possible cancer-causing effects of metallic biomaterials.