Materials Characterization (2019 Edition)
Liquid Chromatography
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Published:2019
Abstract
This article describes high-performance liquid chromatography and ultra-high-performance liquid chromatography that are used to separate and quantify the chemical components in any sample that can be dissolved in a liquid. This includes pharmaceutical drugs, medicinal plant extracts, food constituents, flavors, fragrances, industrial chemicals, pesticides, and pollutants. Readers are introduced to the most commonly employed mode, reverse-phase chromatography, with examples and an exclusive focus on commercially available instruments and consumables. The discussion covers the various processes involved in liquid chromatography, including assessing a separation of sample components, adjusting the mobile phase, choosing the stationary phase, optimizing a separation, preparing real samples, and analyzing complex samples.
Kavita M. Jeerage, Gregory P. Dooley, Liquid Chromatography, Materials Characterization, Vol 10, 2019 ed., ASM Handbook, ASM International, 2019, p 242–253, https://doi.org/10.31399/asm.hb.v10.a0006636
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