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ASM Handbook

Materials Characterization

Edited by
Ruth E. Whan
Ruth E. Whan
Sandia National Laboratories
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ASM International
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Book Chapter

Molecular Fluorescence Spectroscopy

Linda B. McGown
Linda B. McGown
Department of Chemistry, Oklahoma State University
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Page range:
72 - 81


This article provides an introduction to the molecular fluorescence spectroscopy, and discusses the theory of fluorescence and its application to chemical analysis. It provides information on fluorescence that occurs in organic compounds and inorganic atoms and molecules. The article describes the instruments used in the spectroscopy, namely, radiation sources, sample holders, wavelength selectors, detectors, computers, and ratiometric instruments. The practical considerations include solvent effects, corrected spectra, wavelength calibration, temperature, and scattered light. The article also discusses the uses of some special techniques used in molecular fluorescence spectroscopy.

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Linda B. McGown, 1986. "Molecular Fluorescence Spectroscopy", Materials Characterization, Ruth E. Whan

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